Regulation of sheep oocyte maturation using cAMP modulators
نویسندگان
چکیده
منابع مشابه
Regulation of sheep oocyte maturation using cAMP modulators.
Physical removal of mammalian cumulus-oocyte complexes (COCs) from ovarian follicles results in spontaneous resumption of meiosis, largely because of a decrease in cAMP concentrations, causing asynchrony between cytoplasmic and nuclear maturation and decreased oocyte developmental competence. The aim of this study was to modulate cAMP concentrations within ovine COCs to delay spontaneous nuclea...
متن کاملP-64: Anthocyanin Effects on Sheep Oocytes In Vitro Maturation in The Presence of cAMP Modulators.
Background We have previously reported the effect of Anthocyanin and cAMP modulators on maturation and apoptosis of sheep oocytes. In this study, we investigated the reduction capacity and apoptotic gene expression results at MII sheep oocytes. MaterialsAndMethods Good ovine Cumulus-oocyte complexes (COCs) were transferred to Pre-IVM medium, then to two type IVM mediums: 1) 0.1μg/ml Delphinidin...
متن کاملThe regulation of oocyte maturation.
Abbreviations 1-MA 1-methyladenine AC adenylate cyclase cADPr cyclic ADP-ribose CaMKII calcium/calmodulin-dependent kinase DHP 17,20b-dihydro-4-pregnen-3-one ER endoplasmic reticulum ES cells embryonic stem cells FF-MAS follicular fluid meiosis-activating sterol FSH follicle-stimulating hormone GPCR G-protein coupled receptor
متن کاملGlycolytic Metabolites Are Critical Modulators of Oocyte Maturation and Viability
The maturation of an oocyte into an egg is a key step in preparation for fertilization. In Xenopus, oocyte maturation is independent of transcription, being regulated at the level of translation and post-translational modifications of proteins. To identify factors involved in the maturation process we used two-dimensional differential gel electrophoresis to compare the proteome of oocytes and e...
متن کاملHeparin and cAMP modulators interact during pre-in vitro maturation to affect mouse and human oocyte meiosis and developmental competence.
STUDY QUESTION Does heparin ablate the advantageous effects of cyclic adenosine mono-phosphate (cAMP) modulators during pre-in vitro maturation (IVM) and have a deleterious effect in standard oocyte IVM? SUMMARY ANSWER Heparin interrupts energy metabolism and meiotic progression and adversely affects subsequent development of oocytes under conditions of elevated cAMP levels in cumulus-oocyte ...
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ژورنال
عنوان ژورنال: Theriogenology
سال: 2013
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2012.09.020